Part:BBa_K1085035

EstA Strep-tag SpiderSilkSubunitE1

This biobrick contains the EstA SRP sequence attached to the silk subunit E1 with an N-terminal strep-tag.

The Strep-tag, fused at the N-terminal, is used both as a binding tag to use to indicate its production and as a binding component to Streptavidin.

Subunit E1 codes for a spider silk protein that is optimized for maximal expression in Bacillus subtilis 168. The primary goal of the algorithm was to optimize the codon selection based on their availability scores. The secondary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS, and the tertiary goal was to minimize the number of restriction sites.

The amino acid sequence is based on the adapted MaSp2 sequences described in the paper of Brooks et. al. 2008.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 108
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]